Karyotyping of Single Human Chromosomes from Dry Mass Determined by Electron Microscopy

Karyotyping of single human chromosomes from dry mass determined by electron microscopy.

Human chromosomes from cultures of peripheral lymphocytes were prepared for electron microscopy by surface spreading and critical-point drying. Total and partial determinations of chromosomal dry mass were made from contrast in electron microscopic negatives. The distribution of dry weights was analyzed with respect to the Denver-Chicago classifications. There is considerable variability of mas...

Examination of Lung Parenchyma by Electron Microscopy

In this paper many of the principal struc­ture of the Moral Lung were studied with the help of Electron Microscopy. Detailed descrip­tion was given of Granular Pneumocytes, Membranous Pneumocytes, Macrophages, and Alveolar Capillaries. A discussion followed on the form and function of Granular Pneumo­cytes.  Acknowledgements  I am grateful to the Reza Shah Pahlavi Foundation for its generou...

Quantitative karyotyping of human chromosomes by dual beam flow cytometry.

Dual beam flow cytometry of chromosomes stained with Hoechst 33258 and chromomycin A3 has been proposed as a method for quantitative classification of human chromosomes (bivariate flow karyotyping). In this paper we investigate the sources and magnitudes of variability in the mean fluorescence intensities of each chromosome group resolved in bivariate flow karyotypes and study the impact of thi...

Karyotyping human and mouse cells using probes from single-sorted chromosomes and open source software.

Multispectral karyotyping analyzes all chromosomes in a single cell by labeling them with chromosome-specific probes conjugated to unique combinations of fluorophores. Currently available multispectral karyotyping systems require the purchase of specialized equipment and reagents. However, conventional laser scanning confocal microscopes that are capable of separating multiple overlapping emiss...

A Comparative Study and Karyotyping of Chromosomes of Hymenolepis nanafraterna from Mice and Rats